TL;DR
Research methodology for investigating SS-31 cardiolipin binding, mitochondrial membrane preservation, and bioenergetics in disease models.
Research use only. This guide summarizes published research methodology for educational purposes. All Aureum Peptides products are sold strictly for in-vitro laboratory research.
Cardiolipin Binding Characterization
Surface plasmon resonance (SPR) or isothermal titration calorimetry (ITC) studies quantify SS-31 affinity for cardiolipin-containing liposomes. Binding is highly selective — a defining pharmacological feature.
Mitochondrial Membrane Potential (ΔΨm)
TMRM or JC-1 dye-based flow cytometry and confocal imaging of isolated mitochondria or whole cells under oxidative challenge. SS-31 treatment preserves ΔΨm in published studies.
Cristae Architecture via Electron Microscopy
Transmission electron microscopy of cardiac or renal mitochondria in ischemia-reperfusion models — SS-31 treatment associated with preserved cristae architecture and reduced swelling.
Seahorse Bioenergetics Assays
Oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) measurements in cell cultures exposed to SS-31 under basal, stressed, or aged conditions.
Clinical Translation Context
SS-31 has advanced through clinical-stage research/advanced translational research contexts for mitochondrial myopathies and other indications — researchers should distinguish research-use-only SS-31 from pharmaceutical Elamipretide product.
Research-Grade Material from Aureum
Aureum Peptides provides research-grade peptides with ≥99% HPLC-verified purity and batch-specific Certificate of Analysis. Same-day dispatch on qualified orders placed before 2:00 PM EST.
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Disclaimer: Educational content for qualified researchers. Not research information. No therapeutic claims. Peptides not approved by FDA for human use. For in-vitro laboratory research purposes only.
Last reviewed: 2026-04-21
