Follistatin-344 is the full-length 344-amino acid isoform of human follistatin, a glycoprotein that binds and neutralizes activins, particularly activin A. It is a key regulator of the activin/myostatin signaling pathway. Follistatin has been studied in muscle biology, reproductive biology, and metabolic research.
Biochemical Characteristics
Specifications
Molecular Formula
C1539H2380N422O462S36
Molecular Weight
~38000 g/mol
CAS Number
80449-31-6
Follistatin-344 is a ~38 kDa glycoprotein containing three follistatin domains (FSD1-3) and a heparin-binding domain. It binds activin A with high affinity (Kd ~50-200 pM), preventing activin from engaging its type II receptors (ActRII/ActRIIB).
The 344-residue isoform includes a C-terminal heparin-binding sequence that allows cell-surface association, distinguishing it from the shorter FST288 isoform.
Research Applications
• Myostatin/activin biology: Studies on TGF-β superfamily signaling and muscle mass regulation.
• Reproductive biology: Research on FSH regulation and follicular development.
• Metabolic research: Studies on follistatin's effects on glucose homeostasis and brown adipose tissue.
• Muscle biology: Investigations into follistatin's effects on satellite cell proliferation and differentiation.
Form & Analytical Testing
Form: Lyophilized protein, 1mg per vial
Appearance: White powder
Solubility: Soluble in sterile water or PBS with carrier protein
Testing includes SDS-PAGE, MS, endotoxin, sterility, and biological activity assay.
Article Author
DS
Article Author
Dr. Sarah Chen, Ph.D.
Biochemistry researcher specializing in growth factor and protein biology.
This content was prepared for informational purposes based on published preclinical research. It does not constitute medical advice or product efficacy claims.
Citations
1
Rodino-Klapac LR, et al. "Inhibition of myostatin with emphasis on follistatin as a therapy for muscle disease." Muscle Nerve. 2009;39(3):283-96. PubMed
2
Phillips DJ, de Kretser DM. "Follistatin: a multifunctional regulatory protein." Front Neuroendocrinol. 1998;19(4):287-322. PubMed
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Properties
Molecular Formula
C1539H2380N422O462S36
Molecular Weight
~38000 g/mol
98.0%
HPLC Purity
Target: ≥98%
1mg
Peptide Content
~38 kDa
Mol. Weight (MS)
<0.1 EU/μg
Endotoxins
Pass (USP <71>)
Sterility
Janssen Analytical Laboratories
Salt Lake City, UT 84101
Verified
Test
Target
Result
SDS-PAGE Purity
u226595%
98% ✓
Mass Spectrometry
38 u00b1 2 kDa
38 kDa ✓
Endotoxin (LAL)
<0.1 EU/u03bcg
<0.05 EU/u03bcg ✓
Sterility (USP <71>)
No growth
Pass ✓
Biological Activity
Activin A neutralization
ED50 < 200 ng/mL ✓
Temperature
Lyophilized: -20°C to -80°C, protected from light
Reconstituted: 2-8°C, use within 7 days
Shelf Life
12 months lyophilized at -80°C
7 days reconstituted at 2-8°C
Handling
Reconstitute with sterile water or 0.1% BSA/PBS.
Aliquot immediately — do not refreeze.
Handle as protein (sensitive to surface adsorption).
Use low-binding tubes.
Frequently Asked Questions
Follistatin 344 1mg is verified at ≥98.0% purity via reverse-phase HPLC analysis. Each batch also undergoes ESI mass spectrometry for identity confirmation, LAL endotoxin testing (<0.1 EU/mg), and USP <71> sterility testing. A batch-specific Certificate of Analysis (COA) is included with every order.
Add bacteriostatic water (0.9% benzyl alcohol) slowly to the vial wall. Gently swirl to dissolve — do not shake or vortex. Solubility: Soluble in sterile water or PBS with carrier protein. Reconstituted peptide should be stored at 2-8°C and used within 30 days. For single-use applications, sterile water may be substituted (no preservative, use immediately).
Lyophilized (powder): Store at -20°C, protected from light and moisture. Shelf life: 24 months. Reconstituted (solution): Store at 2-8°C, use within 30 days. Critical: Allow the vial to reach room temperature before opening to prevent moisture condensation inside the vial. Avoid repeated freeze-thaw cycles (max 3 recommended).
The CAS registry number for Follistatin 344 1mg is 80449-31-6. Molecular weight: ~38000 g/mol. Molecular formula: C1539H2380N422O462S36. Full chemical characterization data including PubChem identifiers, SMILES notation, and InChIKey are available on the Chemical Properties tab above.
Yes. Every batch of Follistatin 344 1mg is independently tested by Janssen Analytical Laboratories (ISO 17025-accredited). Testing includes HPLC purity analysis, mass spectrometry identity confirmation, LAL kinetic turbidimetric endotoxin assay, and USP <71> sterility testing. Full results are provided in the batch-specific COA. Additional documentation is available on the COA/HPLC/MS and 3rd Party Testing tabs above.
Orders placed before 2:00 PM EST ship same day via cold-chain logistics with gel ice packs to maintain product integrity during transit. Most domestic orders arrive within 2-4 business days. Full tracking is provided. All vials are sealed under inert atmosphere and packaged to prevent damage.
Yes. Tiered pricing is applied automatically at checkout: 2+ units at 10% off, 3+ units at 15% off, and 5+ units at 20% off. For institutional or bulk research inquiries (10+ vials), contact us at contact@aureumpeptides.com for custom pricing.
Compatibility depends on your specific experimental design. Follistatin 344 1mg is provided as a single-compound lyophilized preparation for maximum flexibility. When using multiple compounds, reconstitute each separately and add to experimental systems independently to avoid potential interactions during reconstitution. Check the "Researchers Also Purchased" section below for commonly paired research compounds.
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