CJC-1295 without DAC (Drug Affinity Complex), also known as Modified GRF 1-29 or Mod GRF, is a synthetic 29-amino acid peptide analog of growth hormone-releasing hormone (GHRH). It was developed with four amino acid substitutions to resist enzymatic degradation by dipeptidyl peptidase IV (DPP-IV), extending its biological half-life compared to native GHRH(1-29).
CJC-1295 No DAC is a tetrasubstituted analog of human GHRH(1-29) with molecular weight 3367.97 g/mol. The four amino acid substitutions at positions 2 (D-Ala), 8 (Gln), 15 (Ala), and 27 (Leu) confer resistance to DPP-IV proteolysis.
Unlike the DAC-conjugated variant, this form does not bind albumin and has a shorter circulating half-life, making it useful for pulsatile GHRH receptor activation studies.
Research Applications
• GHRH receptor biology: Studies have examined CJC-1295's interaction with the GHRH receptor (GHRHR) and downstream somatotroph signaling.
• GH secretion research: Investigations into pulsatile GH release patterns and the synergistic effects with ghrelin-mimetic peptides.
• Metabolic research: Studies examining GH axis modulation and associated effects on lipolysis and nitrogen balance in animal models.
• Neuroendocrine research: Investigations into hypothalamic-pituitary axis feedback mechanisms.
Pathway Context
• GHRHR activation → cAMP/PKA signaling cascade
• Somatotroph cell GH synthesis and pulsatile release
• IGF-1 axis downstream signaling (hepatic)
• Synergistic interaction with ghrelin receptor (GHS-R1a) pathway when co-administered with GHRP analogs
Preclinical Research
Teichman et al. (2006) demonstrated dose-dependent GH release in human subjects following subcutaneous CJC-1295 administration in a Phase I clinical trial.
Alba et al. (2006) showed sustained elevations in GH and IGF-1 levels in healthy volunteers with CJC-1295 (DAC-conjugated form).
Note: These studies used the DAC-conjugated form. Non-DAC research is primarily preclinical.
Form & Analytical Testing
Form: Lyophilized powder, 2mg per vial
Appearance: White powder
Molecular Weight: 3367.97 g/mol
Reconstitution Guide:
- For 1 mg/mL: Add 2.0 mL bacteriostatic water
- For 0.5 mg/mL: Add 4.0 mL bacteriostatic water
- For 100 µg/mL: Add 20.0 mL bacteriostatic water
- Gently swirl until dissolved
Solubility: Soluble in water (~2-5 mg/mL), bacteriostatic water, PBS. May require gentle warming (37°C water bath, 5 min) if slow to dissolve.
Stability: Lyophilized stable 24 months at -20°C. Reconstituted: 14-30 days at 2-8°C.
Note: This is the non-DAC form (no Drug Affinity Complex). It does NOT bind albumin and has a shorter half-life (~30 min) compared to DAC-conjugated CJC-1295 (~8 days). This makes it suitable for pulsatile GHRH receptor studies.
Analytical Testing: HPLC, ESI-MS, LAL endotoxin, USP sterility. COA per batch.
Article Author
DS
Article Author
Dr. Sarah Chen, Ph.D.
Biochemistry researcher specializing in peptide characterization and neuroendocrine peptide research.
This content was prepared for informational purposes based on published preclinical research. It does not constitute medical advice or product efficacy claims.
Citations
1
Teichman SL, et al. "Prolonged stimulation of growth hormone (GH) and insulin-like growth factor I secretion by CJC-1295." J Clin Endocrinol Metab. 2006;91(3):799-805. PubMed: 16352683PubMed
2
Alba M, et al. "Once-daily administration of CJC-1295 normalizes growth in the GHRH knockout mouse." Am J Physiol. 2006;291(6):E1290-4. PubMed: 16822960PubMed
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ALL ARTICLES AND PRODUCT INFORMATION ARE FOR INFORMATIONAL AND EDUCATIONAL PURPOSES ONLY. Products are for in-vitro studies only. Not approved by the FDA. Bodily introduction strictly forbidden. These products are not drugs, supplements, food, or cosmetics and may not be acquired for such purposes.
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Properties
Molecular Formula
C152H252N44O42
Molecular Weight
3367.97 g/mol
Monoisotopic Mass
3366.89
Heavy Atoms
240
H-Bond Donors
40
H-Bond Acceptors
46
Identifiers
PubChem CID
56841945
CJC-1295 (Modified GRF 1-29) 2D Structure
Source: PubChem
99.1%
HPLC Purity
Target: ≥98%
2mg
Peptide Content
3368.0 Da
Mol. Weight (MS)
<0.1 EU/mg
Endotoxins
Pass (USP <71>)
Sterility
Janssen Analytical Laboratories
Salt Lake City, UT 84101
Verified
Test
Target
Result
HPLC Purity
u226598.0%
99.1% ✓
Mass Spectrometry
3368.0 u00b1 2.0 Da
3368.0 Da ✓
Endotoxin (LAL)
<0.1 EU/mg
<0.05 EU/mg ✓
Sterility (USP <71>)
No growth
Pass ✓
Temperature
Lyophilized: -20°C, protected from light
Reconstituted: 2-8°C
Shelf Life
24 months lyophilized at -20°C
30 days reconstituted at 2-8°C
Handling
Allow vial to reach room temperature before opening.
Reconstitute with bacteriostatic water.
Avoid repeated freeze-thaw cycles.
Frequently Asked Questions
CJC-1295 No DAC is verified at ≥99.1% purity via reverse-phase HPLC analysis. Each batch also undergoes ESI mass spectrometry for identity confirmation, LAL endotoxin testing (<0.1 EU/mg), and USP <71> sterility testing. A batch-specific Certificate of Analysis (COA) is included with every order.
Add bacteriostatic water (0.9% benzyl alcohol) slowly to the vial wall. Gently swirl to dissolve — do not shake or vortex. Solubility: Soluble in water (~2-5 mg/mL), bacteriostatic water, PBS. May require gentle warming (37°C water bath, 5 min) if slow to dissolve.. Reconstituted peptide should be stored at 2-8°C and used within 30 days. For single-use applications, sterile water may be substituted (no preservative, use immediately).
Lyophilized (powder): Store at -20°C, protected from light and moisture. Shelf life: 24 months. Reconstituted (solution): Store at 2-8°C, use within 30 days. Critical: Allow the vial to reach room temperature before opening to prevent moisture condensation inside the vial. Avoid repeated freeze-thaw cycles (max 3 recommended).
The CAS registry number for CJC-1295 No DAC is 863288-34-0. Molecular weight: 3367.97 g/mol. Molecular formula: C152H252N44O42. Full chemical characterization data including PubChem identifiers, SMILES notation, and InChIKey are available on the Chemical Properties tab above.
Yes. Every batch of CJC-1295 No DAC is independently tested by Janssen Analytical Laboratories (ISO 17025-accredited). Testing includes HPLC purity analysis, mass spectrometry identity confirmation, LAL kinetic turbidimetric endotoxin assay, and USP <71> sterility testing. Full results are provided in the batch-specific COA. Additional documentation is available on the COA/HPLC/MS and 3rd Party Testing tabs above.
Orders placed before 2:00 PM EST ship same day via cold-chain logistics with gel ice packs to maintain product integrity during transit. Most domestic orders arrive within 2-4 business days. Full tracking is provided. All vials are sealed under inert atmosphere and packaged to prevent damage.
Yes. Tiered pricing is applied automatically at checkout: 2+ units at 10% off, 3+ units at 15% off, and 5+ units at 20% off. For institutional or bulk research inquiries (10+ vials), contact us at contact@aureumpeptides.com for custom pricing.
Compatibility depends on your specific experimental design. CJC-1295 No DAC is provided as a single-compound lyophilized preparation for maximum flexibility. When using multiple compounds, reconstitute each separately and add to experimental systems independently to avoid potential interactions during reconstitution. Check the "Researchers Also Purchased" section below for commonly paired research compounds.
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