AOD-9604 (Anti-Obesity Drug 9604) is a synthetic peptide corresponding to a modified fragment of human growth hormone (hGH), specifically residues 177-191 with an N-terminal tyrosine addition. Developed by Metabolic Pharmaceuticals, it was designed to isolate the lipolytic activity of GH from its growth-promoting effects. AOD-9604 has been studied in preclinical and clinical obesity research.
AOD-9604 has a molecular weight of 1815.08 g/mol. It contains an internal disulfide bond between Cys182 and Cys189 that is essential for biological activity. The peptide reproduces the lipolytic domain of hGH without binding to the GH receptor, and therefore does not promote IGF-1 elevation in preclinical models.
The N-terminal tyrosine is a structural addition to the native hGH 177-191 sequence.
Research Applications
• Lipid metabolism: Studies have examined AOD-9604's effects on lipolysis in adipocyte cell cultures and adipose tissue explants.
• Obesity research: Clinical trials investigated oral AOD-9604 for body weight reduction in obese subjects (Stier et al., 2013).
• Cartilage biology: Research has explored AOD-9604's effects on chondrocyte proliferation and cartilage repair models.
• Metabolic signaling: Studies on AOD-9604's interaction with beta-3 adrenergic receptor-mediated lipolysis.
Pathway Context
• Beta-3 adrenergic receptor pathway interaction in adipocytes
• Lipolytic signaling independent of GH receptor binding
• Non-IGF-1-mediated metabolic effects
• Chondrocyte proliferation pathway (separate from lipolytic activity)
Preclinical Research
Heffernan et al. (2000) demonstrated that the hGH fragment (177-191) stimulated lipolysis in obese mouse models without diabetogenic effects.
Stier et al. (2013) conducted clinical trials of oral AOD-9604 in obese subjects, examining safety and metabolic parameters.
AOD-9604 received GRAS (Generally Recognized as Safe) status from the FDA as a food ingredient in 2014.
Form & Analytical Testing
Form: Lyophilized powder, 5mg per vial
Appearance: White powder
Molecular Weight: 1815.08 g/mol
Reconstitution Guide:
- For 1 mg/mL: Add 5.0 mL bacteriostatic water
- For 2 mg/mL: Add 2.5 mL bacteriostatic water
- Gently swirl until dissolved
Solubility: Soluble in water (~5 mg/mL), bacteriostatic water. Disulfide bond between Cys182-Cys189 is essential for activity — avoid reducing agents (DTT, TCEP, β-mercaptoethanol).
Stability: Lyophilized stable 24 months at -20°C. Reconstituted: 30 days at 2-8°C.
Regulatory Note: AOD-9604 received GRAS (Generally Recognized as Safe) status from the FDA in 2014 as a food ingredient.
Biochemistry researcher specializing in metabolic peptide research.
This content was prepared for informational purposes based on published preclinical research. It does not constitute medical advice or product efficacy claims.
Citations
1
Heffernan MA, et al. "The effects of human GH and its lipolytic fragment (AOD9604) on lipid metabolism following chronic treatment in obese mice and beta(3)-AR knock-out mice." Endocrinology. 2001;142(12):5182-9. PubMed
2
Stier H, et al. "Safety and tolerability of the hexadecapeptide AOD9604 in humans." Growth Horm IGF Res. 2013;23(1-2):20-5. PubMed
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ALL ARTICLES AND PRODUCT INFORMATION ARE FOR INFORMATIONAL AND EDUCATIONAL PURPOSES ONLY. Products are for in-vitro studies only. Not approved by the FDA. Bodily introduction strictly forbidden. These products are not drugs, supplements, food, or cosmetics and may not be acquired for such purposes.
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Properties
Molecular Formula
C78H123N23O23S2
Molecular Weight
1815.08 g/mol
Monoisotopic Mass
1813.84
Heavy Atoms
131
Identifiers
PubChem CID
10270414
AOD-9604 (hGH Fragment 177-191) 2D Structure
Source: PubChem
99.0%
HPLC Purity
Target: ≥98%
5mg
Peptide Content
1815.1 Da
Mol. Weight (MS)
<0.1 EU/mg
Endotoxins
Pass (USP <71>)
Sterility
Janssen Analytical Laboratories
Salt Lake City, UT 84101
Verified
Test
Target
Result
HPLC Purity
u226598.0%
99.0% ✓
Mass Spectrometry
1815.1 u00b1 1.0 Da
1815.1 Da ✓
Endotoxin (LAL)
<0.1 EU/mg
<0.05 EU/mg ✓
Sterility (USP <71>)
No growth
Pass ✓
Temperature
Lyophilized: -20°C, protected from light
Reconstituted: 2-8°C
Shelf Life
24 months lyophilized at -20°C
30 days reconstituted at 2-8°C
Handling
Allow vial to reach room temperature before opening.
Reconstitute with bacteriostatic water.
Avoid repeated freeze-thaw cycles.
Contains disulfide bond; avoid reducing conditions.
Frequently Asked Questions
AOD 9604 is verified at ≥99.0% purity via reverse-phase HPLC analysis. Each batch also undergoes ESI mass spectrometry for identity confirmation, LAL endotoxin testing (<0.1 EU/mg), and USP <71> sterility testing. A batch-specific Certificate of Analysis (COA) is included with every order.
Add bacteriostatic water (0.9% benzyl alcohol) slowly to the vial wall. Gently swirl to dissolve — do not shake or vortex. Solubility: Soluble in water (~5 mg/mL), bacteriostatic water. Disulfide bond between Cys182-Cys189 is essential for activity — avoid reducing agents (DTT, TCEP, β-mercaptoethanol).. Reconstituted peptide should be stored at 2-8°C and used within 30 days. For single-use applications, sterile water may be substituted (no preservative, use immediately).
Lyophilized (powder): Store at -20°C, protected from light and moisture. Shelf life: 24 months. Reconstituted (solution): Store at 2-8°C, use within 30 days. Critical: Allow the vial to reach room temperature before opening to prevent moisture condensation inside the vial. Avoid repeated freeze-thaw cycles (max 3 recommended).
The CAS registry number for AOD 9604 is 221231-10-3. Molecular weight: 1815.08 g/mol. Molecular formula: C78H123N23O23S2. Full chemical characterization data including PubChem identifiers, SMILES notation, and InChIKey are available on the Chemical Properties tab above.
Yes. Every batch of AOD 9604 is independently tested by Janssen Analytical Laboratories (ISO 17025-accredited). Testing includes HPLC purity analysis, mass spectrometry identity confirmation, LAL kinetic turbidimetric endotoxin assay, and USP <71> sterility testing. Full results are provided in the batch-specific COA. Additional documentation is available on the COA/HPLC/MS and 3rd Party Testing tabs above.
Orders placed before 2:00 PM EST ship same day via cold-chain logistics with gel ice packs to maintain product integrity during transit. Most domestic orders arrive within 2-4 business days. Full tracking is provided. All vials are sealed under inert atmosphere and packaged to prevent damage.
Yes. Tiered pricing is applied automatically at checkout: 2+ units at 10% off, 3+ units at 15% off, and 5+ units at 20% off. For institutional or bulk research inquiries (10+ vials), contact us at contact@aureumpeptides.com for custom pricing.
Compatibility depends on your specific experimental design. AOD 9604 is provided as a single-compound lyophilized preparation for maximum flexibility. When using multiple compounds, reconstitute each separately and add to experimental systems independently to avoid potential interactions during reconstitution. Check the "Researchers Also Purchased" section below for commonly paired research compounds.
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